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All participating dogs were subjected to a clinical examination and blood collection.

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Sensitivity and specificity are provided for each of the techniques used.

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All participating dogs were subjected to a clinical examination and blood collection.

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Blood cell counts consistently revealed severe regenerative anaemia and thrombocytopenia in dogs with piroplasmosis due to T.

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PCR and sequencing was used as the gold standard reference method to identify small piroplasms [ 2939 ].

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Currently, there are no diagnostic tools to distinguish between the different piroplasms in routine veterinary practice and their detection by microscopy in red blood cells is still the only method available to practitioners.

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Haematology and biochemistry The following variables were determined using an automated blood analyser Sysmex XTi, Roche Diagnostics, Spain:

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