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All subjects were asked for a minimum of 2 days of sexual abstinence.

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Sperm morphology, motility and concentration in fertile and infertile men.

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Classification order hierarchy is standardized and sperm characteristics are defined.

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Differences in semen parameters among various subgroups normozoospermia, asthenozoospermia, oligozoospermia and oligoasthenozoospermia were assessed by one-way ANOVA.

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Our novel dichotomous key provides a rational decision framework for a sperm morphology classification algorithm.

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How reliable are results from the semen analysis?

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We observed a good level of agreement between the observers for both sperm morphology assessment criteria.

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Notes None declared.

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However, Who classification for sperm morphology, in one study on 54 semen samples from eight fertile men and 46 subfertile patients using liquefied and washed samples stained with Diff-Quik and Papanicolaou stains, a very high degree of agreement inter-observer variability for liquefied and washed samples of 0.

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